rabbit anti p mlc (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit anti p mlc
Rabbit Anti P Mlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+p+mlc/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/bio_rxiv__64898__2025__12__20__695343-132-27-29
Average 96 stars, based on 1390 article reviews

rabbit anti p mlc - by Bioz Stars, 2026-09

96/100 stars

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Saline:

Article Title: Taxane-Induced Conformational Changes in the Microtubule Lattice Activate GEF-H1-Dependent RhoA Signaling
Article Snippet: Lysates were run on 5-16% acrylamide gels and transferred, to PVDF membrane (0.45 μm pore size, Merck Millipore, IPVH00010) or nitrocellulose membrane (0.2 μm pore size, Amersham, 10600006) in the case of p-MLC, using wet transfer at 37 V at 4 °C overnight. .. Membranes were blocked using 5% BSA in Tris buffered saline with 0.1% Tween 20 (TBST) for 2-8 hours then incubated in primary antibody diluted in blocking buffer, rabbit anti-p-MLC (Cell Signaling, 3671S, dil 1:250) or rabbit anti-GEF-H1 (Abcam, ab155785, dil 1:1000) overnight or mouse anti-actin (Sigma, MAB1501, dil 1:4000) for 1 hour. .. HT1080, U2OS and HEK293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Capricorn Scientific) supplemented with 10% Fetal Bovine Serum (FBS; Corning) and 100U/mL Penicillin and 100 μg/mL Streptomycin (1% Pen Strep; Sigma).

Incubation:

Article Title: Jdp2 is a spatiotemporal transcriptional activator of the AhR via the Nrf2 gene battery
Article Snippet: .. After blocking with 5% goat serum in PBS, cells were stained overnight with rabbit anti-p-MLC (1:50, Cell Signaling Technology, Danvers, MA, USA) at 4 °C, washed thoroughly with PBS, and incubated with Alexa Fluor 594 goat anti-rabbit secondary antibody (1:400, Invitrogen) at room temperature for 60 min. To label actin stress fibers, actin was counterstained in green using Alexa Fluor 488-labeled phalloidin (1:80, Invitrogen). ..

Article Title: Up-regulation of Rac1 in the bronchial smooth muscle of murine experimental asthma.
Article Snippet: .. The membranes (polyvinylidene difluoride membranes) were incubated with the following primary antibodies: rabbit anti-p-MLC (Ser19, 1:1000 dilution; Cell Signalling Technology, MA, USA), rabbit anti-MLC (1:1000; Cell Signalling Technology, MA, USA), rabbit anti-MYPT1 (1:1000, Cell Signalling Technology, MA, USA), rabbit A cc ep te d A rt ic le This article is protected by copyright. .. All rights reserved. phosphor-MYPT1 (Thr696) antibody (1:1000, Cell Signalling Technology, MA, USA), rabbit phosphor-MYPT1 (Thr853, 1:1000, Cell Signalling Technology, MA, USA), or anti-Rac1 (1:1000, Merck Millipore, MA, USA).

Article Title: Conditional deletion of Rcan1 predisposes to hypertension-mediated intramural hematoma and subsequent aneurysm and aortic rupture
Article Snippet: .. Samples were incubated overnight with Cy3-conjugated monoclonal anti-SMA (1:500, C6198, Sigma) and rabbit anti-p-MLC (1:50, #3671, Cell Signaling). .. Secondary antibody was AlexaFluor647-conjugated goat anti-rabbit (1:500; A-21245; BD Pharmingen).

Article Title: Conditional deletion of Rcan1 predisposes to hypertension-mediated intramural hematoma and subsequent aneurysm and aortic rupture
Article Snippet: .. Samples were incubated with the following antibodies for immunofluorescence: Cy3-conjugated monoclonal anti-SMA (1:500, C6198, Sigma), rabbit anti-cd31 (1:50, ab28364, Abcam), and rabbit anti-p-MLC (1:50, #3671, Cell Signaling). .. For permeability assays, cryosections (8 μm) were obtained, blocked with 10% goat serum plus 2% BSA in PBS for 1 h, and then incubated with hamster anti-cd31 (1:200; MAB1398Z; Millipore) and rabbit anti-p-MLC primary antibodies.

Article Title: Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.
Article Snippet: .. Animal caps of the bisected embryos were incubated overnight at 4°C in rabbit anti-P-MLC (1:100; #3671; Cell Signaling Technologies) in blocking solution, washed three times with blocking solution, and incubated overnight at 4°C in goat anti-rabbit Alexa Fluor 488 IgG (1:200; #A11008; Thermo Fisher Scientific) and Alexa Fluor 647 phalloidin (1:1,000). ..

Blocking Assay:

Staining:

Immunofluorescence:

Article Title: Conditional deletion of Rcan1 predisposes to hypertension-mediated intramural hematoma and subsequent aneurysm and aortic rupture
Article Snippet: .. Samples were incubated with the following antibodies for immunofluorescence: Cy3-conjugated monoclonal anti-SMA (1:500, C6198, Sigma), rabbit anti-cd31 (1:50, ab28364, Abcam), and rabbit anti-p-MLC (1:50, #3671, Cell Signaling). .. For permeability assays, cryosections (8 μm) were obtained, blocked with 10% goat serum plus 2% BSA in PBS for 1 h, and then incubated with hamster anti-cd31 (1:200; MAB1398Z; Millipore) and rabbit anti-p-MLC primary antibodies.

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